[reBane]

With for example 32 human players and 22 bots for standard pop files your values should be:

add these startup options
-unrestricted_maxplayers -maxplayers 54
54 = is humand player count + bot player count

in the server config set
tf_mvm_defenders_team_size 32
tf_mvm_max_connected_players 32

That's like all i had to do